Synchronous Determination of Creatine Compounds, Adenine Nucleotides, Hypoxanthine and Inosine with HPLC
- VernacularTitle:HPLC同步测定肌酸、磷酸肌酸、腺苷酸及次黄嘌呤类物质
- Author:
Yuechun ZHU
- Publication Type:Journal Article
- Keywords:
HPLC;
Creatine compounds;
Inosine Adenine nucleotides;
Hypoxanthine
- From:
Journal of Kunming Medical University
1989;0(01):-
- CountryChina
- Language:Chinese
-
Abstract:
Synchronous determination of creatine Compounds, adenine nucleotides, hypoxanthine and inosine with reversed—phase HPLC was studied. The distinct separation was achieved within 10 minutes at 210nm using radial—PAK C_(18) Cartridge column under an isocratic elution of 220mmol/L K_2HPO_4/KH_2PO_4, mmol/L TBA and 13% methanol. This method proved to be reproducible, simple and accurate.
