The comparison of activities of low density lipoprotein receptors in human fetal organs and tissues
- VernacularTitle:人胎儿各器官组织低密度脂蛋白受体活性的比较
- Author:
Ming LI
;
Haijiang CAI
- Publication Type:Journal Article
- Keywords:
Fetus, human;
Receptors, LDL
- From:
Chinese Journal of Pathophysiology
1986;0(01):-
- CountryChina
- Language:Chinese
-
Abstract:
The activities of low density lipoprotein (LDL) receptors in 15 organs and tissues from 5 human fetuses of the ages of 22 to 34 weeks were determined using a membrane filter assay of the specific binding of ~(125)I-LDL to tissue homogenates. The results showed that the adrenal cortex (465 ng/mg protein) had the highest activity of ~(125)I LDL specific binding seven times than that of the medulla. Adipose tissue (214 ng/mg protein) and liver (102 ng/mg protein) ranked second. Relatively high bindings were also observed in the skeletal muscle (89 ng/mg protein), brain (65 ng/mg protein), kidney (60 ng/mg protein) and spinal cord (56ng/mg protein). It was found that the activity of LDL receptors in the central nervous system of human fetus was higher than that of cows, of human adultsand of humam fetuses of 16 to 20 weeks as reported by other investigators. Relatively high level of LDL receptor mRNA in human fetal brain was also found by ~(32)P-cDNA probe hybridization analysis. It is suggested that the growing and developing central nervous system of human fetus reqiresmore cholesterol. In addition, We also observed preliminarily that the tendency of LDL receptors activities in liver and adrenal gland cortex were gradually inereased with fetal age.
