DETERMINATION OF CARBOXYHEMOGLOBIN IN BLOOD BY TWO-WAVELENGTH SECOND-DERIVATIVE SPECTROPHOTOMETRY
- VernacularTitle:二阶导数光谱双波长法测定血中碳氧血红蛋白饱和度的研究
- Author:
Xixuan ZHANG
;
Chuichang FAN
;
Jian YU
- Publication Type:Journal Article
- Keywords:
COHb concentration;
CO poisoning;
Second-derivative spectrum;
Two-wavelength
- From:
Chinese Journal of Forensic Medicine
1988;0(04):-
- CountryChina
- Language:Chinese
-
Abstract:
A new method of determination of COHb is reported in this paper by two- wavelength second-derivative spectrophotometry.Blood was diluted with a solution of THAM containing Na_2S_2O_4.The second-derivative spectrum was measured in the region of 500-600 nm and the derivative absorbance differerce ?Ax was deter mined at the wavelengths of 564 nm and 576 nm.Then the solution was satura- ted with CO gas.The second-derivative spectrum of COHb was measured in the same region.The derivative absorbance difference ?A_(100) was determined at the same wavelengths.The COHb in blood was calculated by the ratio of ?Ax to ?A_(100).The results of determination of COHb in standard blood samples were in good agreement with the theoretical values.
