EXAMINATING THE SPERM ANTIGEN IN SEMINAL FLUID AND SEMINAL STAIN USING ELISA METHOD
- VernacularTitle:用ELISA检测精液及精斑中精子抗原的研究
- Author:
Yaqing DUAN
;
Jinghua XU
;
Xiaofeng WANG
;
Guanshi ZHANG
;
Yuxin LI
- Publication Type:Journal Article
- Keywords:
Monoclonal antibody against human sperm;
ELISA sperm antigen;
Seminal fluid;
Seminal stain
- From:
Chinese Journal of Forensic Medicine
1988;0(04):-
- CountryChina
- Language:Chinese
-
Abstract:
The sperm antigens were examined using the method of ELISA and specific monoclonal antiboby against human sperm.The results of ELISA method of 10 fresh seminal fluid samples and 15 seminal stains showed 100% positive rate. Even diluted the fresh seminal fluid to 10~6 times of seminal stains extract to 51?0~5 times,both were positive also.However,it was negtive for testing other specimens such as saliva stains,urine stains,colostrum stains,sweat stains, vaginal secretion stains and human seminal stains after vesoligation. The result certificate that using specific antibody to the sperm,the ELISA method is high sensitive and specific for the sperm antigen assay.
