Establishment of a model of transplantable human undifferentiated cecum adenocarcinoma by injection of cultured cells into nude mice and its biological characteristics
- VernacularTitle:用人盲肠未分化腺癌细胞系建立的裸鼠移植瘤模型及其生物学特性
- Author:
Zongxian ZHANG
;
Yiyin ZHANG
- Publication Type:Journal Article
- Keywords:
Adenocarcinoma, cecum;
Mice, nude: Transplantation
- From:
Chinese Journal of Pathophysiology
1989;0(05):-
- CountryChina
- Language:Chinese
-
Abstract:
A model of transplantable human undifferentiated cecum adenocarcinoma in Balb/C nude mice from the cell line maintained in vitro was established and was named NB-93, 26 sub-transplantations were performed. The rate of success of transplantation was 96.2%. The longest life span of tumor bearing nude mouse was 246 days and its mid-point time was 108 days. Pathohistology showed that the structure of the transplantable tumor was similar to that of the original human tumor. The tumor retained the function of secreting CEA. Chromosomal analysis showed that the tumor cells had a malignant karyotype. The tumor tissue, after being frozen in liquid nitrogen, could be rehabilitated and successfully retransplanted in nude mice again. The model seems to be a useful tool for further study of human undifferentiated cecum adenocarcinoma.
