Effects of ascorbic acid and cortisol on the antigen-presenting function (APF) of normal human monocytes
- VernacularTitle:维生素C、皮质醇对正常人外周血单核细胞抗原提呈功能的影响
- Author:
Kexiang FEI
;
Xinwei FENG
- Publication Type:Journal Article
- Keywords:
Ascorbic acid;
Hydrocortisone;
Monocytcs;
T lymphocytes.
- From:
Chinese Journal of Pathophysiology
1986;0(04):-
- CountryChina
- Language:Chinese
-
Abstract:
Mononuclear cells obtained from heparinized fresh venous blood of healthy male blood donors by means of Ficoll-Hypaque densitz gradient centrifugation were subjected to petri dish adhesion and nylon-wool-column separation to harvest monocytes (Mon) and T lymphocytes (T) high purity, respectively. APF of Mon, as reflected by ~3H-TdR incorporated T proliferation, was assayed after the Mon had been preincubated with PPD (purified protein derivative, end concentration 9.25 ?g/ml), PPD+ascorbic acid, PPD+cortisol, and PPD+ascorbic acid+cortisol for 24 hours at 37℃, in a humidified incubator containing 5% CO_2. It was shown that APF of the Mon was markedly enhanced by ascorbic acid in a concentration of 3.75mM (P0.05).
