ISOLATION AND ANALYSIS OF SINGLE HAIR KERATIN
- VernacularTitle:单根毛发角蛋白的分离与分析
- Author:
Xuemin HE
- Publication Type:Journal Article
- Keywords:
Hair keratin;
SDS-PAGE;
Low-sulphur protein;
High-sulphur protein
- From:
Chinese Journal of Forensic Medicine
1986;0(02):-
- CountryChina
- Language:Chinese
-
Abstract:
The protein components from single hair(1～5 cm in length)have been analysedby using mini SDS-gradient polyacrylamide gel electrophoresis(5.0～17.0%).Thekeratins from hairs of human,pan,comopithecus,hylobates,oryctolagus and caviawere compared.Keratin in human hair differed from the animal hairs in the lowmolecular weight components.The low-sulphur protein and high-sulphur proteinfrom hairs were determined by means of N-(3-pyrene)maleimide labelled hair proteins.The molecular weight of the former was within the range of 45,000～52,000 daltonwhile that of the latter within the range of
