Changes in Hepatocyte DNA Content at Various Stages of Carcinogenesis Induced by 3′-Methyl-4,4′-Dimethylaminoazobenzene
- VernacularTitle:Ⅱ.3′-甲基奶油黄诱癌不同阶段的肝细胞DNA含量变化
- Author:
Zongji ZHANG
- Publication Type:Journal Article
- Keywords:
Rat;
Hepatocytes;
DNA;
3′-Methyl-4;
4′-dimethy;
aminoazobenzene
- From:
Journal of Kunming Medical University
1990;0(02):-
- CountryChina
- Language:Chinese
-
Abstract:
The nuclear DNA content of rat hepatocytes at different stages of hepatocarcinogenesis was measured on cytologic smears using microspe-ctrophotometer at wavelength of 560 nm. A significant reduction in the ratio of tetraploid to diploid cells was induced by oral admimistration of 0.06% 3'-methyl-4,4'-dimethylaminoazobenzene(3'-Me-DAB)for 2 months.Those rats treated with 0.06% 3'-Me-DAB for 7 months had a permanently de- pressed ratio of tetraploid:diploid.The tumor cells at the early stage of carcinogenesis and the percancerous hepatocytes were diploid in pattern.We suggest that the selective outgrowth of diploid cell population would significantly increase the likelihood of developing further to- wards cancer.It might be an important marker of cell transformation.The DNA content and pathomorphology of hepatoma might objectively reflect its malignity.
