A simple assay for LDL receptor activities in human periphral lymphocytes
- VernacularTitle:外周血淋巴细胞LDL受体活性测定新法
- Author:
Jianxin YANG
;
Haijiang CAI
- Publication Type:Journal Article
- Keywords:
Lipoproteins, LDL;
Atherosclerosis;
Hypercholesterolemia, familial
- From:
Chinese Journal of Pathophysiology
1986;0(03):-
- CountryChina
- Language:Chinese
-
Abstract:
Periphral lymphocytes suspended in RPMI-1640 were incubated in plates of 96 wells for 96 hours with 50 ?g/ml of PHA, various concentrations of LDL-ch and0.6 ?M of mevinolin (experimental) or equal volume of its solvent-dimethyl sulfoxide (control). 6 hours before harvesting cells, 0.5?Ci of ~3H-TdR was added to each well, then cells were harvested and their cpm counted. The LDL receptor activity was expressed as mevinolin-mediated lymphocyte proliferation inhibition rate which can be calculated by the following equation:Percent Inhibition Rate=(1-cpm exp./cpm con.)100%.It was found that the lymphocyte proliferation inhibition rate at 5?g/ml of LDL-ch was less than 20% for normal and non-FH hyperlipidemic subjects, 25-55% for FH heterozygotes and over 60% for FH homozygotes. Thus, FH patients were distinguished from normal subjects and non-FH hyperlipidemic patients.
