The effect of Radix Sal viae Miltiorrhizaon the inhibitory activity of vascular smooth muscle cells to the plasminogen activators secreted by endothelial cells
- VernacularTitle:丹参对血管平滑肌细胞抑制内皮细胞分泌的纤溶酶原激活物活性的影响
- Author:
Shenlan MAO
;
Caiying ZHANG
- Publication Type:Journal Article
- Keywords:
Muscle, smooth;
Cells;
Endothelium;
Plasminogen activators
- From:
Chinese Journal of Pathophysiology
1986;0(02):-
- CountryChina
- Language:Chinese
-
Abstract:
Cultured porcine endothelial cells (EC) produce and secrete plasminogen activators (PA). If the serum free medium conditioned by vascular smooth muscle cells (SMC-CM) mixed with the medium conditioned by endothelial cells (EC-CM), the PA activities of the latter decreased significantly. Coeultivation of EC with SMC inhibited 70.7% PA activity of EC. Gel eletrophoretic analysis (SDS-PAGE) of SMC-CM followed by reverse fibrin autography demonstrated PA inhibitory activity in the molecular weight range of 49, 000-52, 000 similar to those identified by Laug (1985).In this study it was also investigated the effect of a Chinese herbal medicine-Radix Salviae Miltiorrhizae (RSM) on the inhibitory activity. SMC were incubated with RSM (0.1% in M-199, the final concentration was 1.5mg/ml) for 24hr. The SMC-CM collected before and after RSM treatment were mixed with EC-CM and urokinase respectively. The results show that the residual PA activities in EC-CM or urokinase samples mixed with SMC-CM treated with RSM were significantly higher than that mixed with SMC-CM without treatment with RSM. SMC-CM neutralized the PA activities in EC-CM or urokinase samples in a concentration dependent manner (P
