Effect of external free radical generating system on electrophysiologic characteristics of rat papillary muscle cells
- VernacularTitle:外源性自由基对大鼠心肌细胞电生理活动的影响
- Author:
Zhongfang LAI
;
Shanggong CHEN
- Publication Type:Journal Article
- Keywords:
Free radicals;
Action potentials;
Arrhythmia;
Catalase
- From:
Chinese Journal of Pathophysiology
1986;0(01):-
- CountryChina
- Language:Chinese
-
Abstract:
Standard microelectrode technique was used to study the effect of external free radical generating system (EFR) on action potential of papillary muscle cells of 48 Wistar male rats. EFR were produced from activated oxygen systems: including hydrogen peroxide (H_2O_2), hydroxyl (OH~-) and superoxider (O_2~-). The experiment was divided into six groups: a). control, b). OH~-, c). O_2~-, d). H_2O_2, e). OH~-+catalase(CAT, 150U/ml, f). H_2O_2+CAT and the data were analysed on-line by a computer (Apple Ⅱ Doctor-851). The results showed: (1). a highly significant reduction in action potential amplitude in group OH~- (from 110.5?11.8mV to 98.0?10.4mV), H_2O_2 (from 112.3?13.2mV to 99.0?13.6mV) and O_2~+ (from 117.2?14.5mV to 103.8?13.2mV). (2). a significant decrease in maxium rate of depolariztion (Vmax) respectively (P
