An improved ELISA for Detection of Monoclonal Antibodies to Human Hepatocellular Carcinoma
- VernacularTitle:检测人肝癌单克隆抗体的改良ELISA法
- Author:
Xiaoyun ZHU
- Publication Type:Journal Article
- Keywords:
Improved ELISA;
MAbs;
Screening;
Liver cancer
- From:
Journal of Kunming Medical University
1988;0(03):-
- CountryChina
- Language:Chinese
-
Abstract:
Instead of poly—I—lysine, the microtitre plate is in turn pretreated with concentrated sulphuric acid and 100?g/ ml protamine. The living target cells are well attached to the plate. Fixed with 0.025—0.05% glutaraldehyda, the target cells are suitable for screening monoclonal antibodies by ELISA. This assay is specific, sensitive and well reproductive. It is a useful method for screening large scale spent supernatant of hybridomas and ascites at less expense and time. On the other hand, this method can be also used to detect cellular antigens with either MAbs or conventional antibodies.
