Preliminary studies on antigenic modulation induced by monoclonal antibody
- VernacularTitle:单克隆抗体诱发肿瘤抗原调变的初步研究
- Author:
Suyun WU
;
Gansheng WEN
- Publication Type:Journal Article
- Keywords:
Antibodies, monoclonal;
Antigens;
Cervix neoplasms;
Mice
- From:
Chinese Journal of Pathophysiology
1986;0(03):-
- CountryChina
- Language:Chinese
-
Abstract:
The TD。 of mouse uterine cervical carcinoma (U_(14)) were inoculated subcutaneously in syngenic mice. After that experimental mice were administered diverse doses of monoclonal anti-U_(14) antibody (AU_(14-1)). It was found that within a 267 day interval after the tumor cell inoculations, all control animals were tumor free with tumor free survival, but 86% (6/7)and 88% (7/8)of experimental mice that had been treated with high dose AU_(14-1) showed progressive tumor growth at the inoculation site and died of systemic tumor disease. These results indicate immune enhancement of AU_(14-1) on U_(14) cells. AU_(14-1) was used to study the response of U_(14) cells to specific anti (?)dy in vitro. The results demonstrate that AU_(14-1) induces antigenic modulation of U_(14) cells, which is shown to be a loss of AU_(14-1) antibody and U_(14) antigen from the surface membrane of these cells as determined by indirect membrane immunofluorescence assay. This suggested that antigenic modulation may be proposed as a mechanism by which cervical cancer cells escape monoclonal antibody therapy.
