“Ischemic damage” of cultured cerebral neurons
- VernacularTitle:体外培养的中枢神经细胞的“缺血”性损害
- Author:
Tianyou WANG
- Publication Type:Journal Article
- Keywords:
Cerebral ischemia;
Neurons;
In vitro;
Culture media
- From:
Chinese Journal of Pathophysiology
1989;0(05):-
- CountryChina
- Language:Chinese
-
Abstract:
The dissociated monotypic nerve cell culture was established using 8 day chick embryonic cerebral hemispheres. On the 6th day in vitro, the culture medium was substituted by Earle's ballanced salt solution without glucose, then the cells were incubated in an atmosphere of 95% nitrogen and 5% CO_2. After 1 to 4 hours, increasing number of nerve cells became swollen, lost their character of optical refraction under phase contrast microscope, and were stained with trypan blue. Their leakage of LDH increased and their ability of high affinity uptake of GABA decreased. The reduction of GABA uptake took place earlier and more remarkable than that of other parameters. No significant change was observed if the ceils were incubated with glucose but without oxygen or with oxygen but without glucose. The above mentioned alterations were similar to nerve cell changes following cerebral ischemia in vivo. This model in vitro is useful in the study of ischemic nerve cell damage, their recovery and the protective effect of drugs.
