Studies on the purification of rabbit monocyte pyrogen
- VernacularTitle:内生致热原的纯化实验研究
- Author:
Zhiqiang LIU
- Publication Type:Journal Article
- Keywords:
Fever;
Pyrogens;
Chromatography;
Isoelectric focusing
- From:
Chinese Journal of Pathophysiology
1986;0(04):-
- CountryChina
- Language:Chinese
-
Abstract:
Crude monocytic endogenous pyrogens from rabbits' blood were further purified by means of ultrafiltration membranes. The sterile pyrogen obtained was passed through gel filtration on a 1.5 by 100cm G-75 Sephadex column, and subje- cted to a 1.5 by 60cm ion-exchange chromatography on QAE Sephadex or subjected to isoelectric focusing. Under our experimental conditions high concentration of monocyte pyrogen could be obtained. In above described method used two pyrogen peak whose specific activity was 0.5—3.0ug, caused fever up to 0.6℃ in rabbits. In addition, isoelectric focusing column employed indicated isoeletric points of specific activity protein that induced fever were PI 5.1 and 3.6 respectively.
