Changes in canine leukocytic glucocorticoid receptors during endotoxin shock
- VernacularTitle:内毒素休克狗白细胞糖皮质激素受体的变化
- Author:
Feng LI
- Publication Type:Journal Article
- Keywords:
Leukocytes;
Dexamethasone;
Receptors, glucocorticoid;
Shock, septic
- From:
Chinese Journal of Pathophysiology
1986;0(04):-
- CountryChina
- Language:Chinese
-
Abstract:
We studied the changes in canine leukocytic glucocorticoid receptors during endotoxin shock. Blood samples for analysis were collected and leukocytes were isolated Just prior to, 2 and 6 hours after endotoxin administration. Employing ~sH-Dexamethasone (~3H-Dex) as a ligand, we studied ~3H-Dex specific binding of the leukocytes in dogs and their changes during endotoxin shock. Results from two groups (anesthetized and conscious) showed that the specific binding of the leukocytes decreased significantly in both groups 2 hours after endotoxin administration, but they tended to return to normal level by 6 hours. We also studied the changes of GCRS after cndotoxin injection by Scatchard analysis in conscious dogs. The apparent binding capacity (Ro) seems to unchange but the apparent equilibrium dissociation const ant (Kd) increased significantly 6 hours after endotoxin injection, suggesting that the affinity of GCRs decreased. It may be suggested that perturbations in glucocorticoid hormone action at the receptor level might bc involved in the pathogenesis of cndotoxin shock.
