Studies on leukemogenic activity of cell-free extract of mouse SRS-82 cell line
- VernacularTitle:SRS-82细胞株的无细胞提取液的致白血病活性研究
- Author:
Li CHENG
- Publication Type:Journal Article
- Keywords:
Leukemia;
Viruses;
Cell line
- From:
Chinese Journal of Pathophysiology
1986;0(01):-
- CountryChina
- Language:Chinese
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Abstract:
Ultrastructural studies of SRS-82 cell line demonstrated certain morphologic characteristics of lymphocytes and the presence of intracistenal type A virus in the cytoplasm. Inoculation of cell-free extract from SRS-82 cell line into 24 newborn mice of Kunmin strain, leukemias were induced in 15 mice (62.5%). Among them 14 are lymphocytic (14/15) and only one is myelocytic (1/15). The average induction period was 169 days. Furthermore, on transfer of ultracentifugation precipitate suspension of SRS-82 culture supernate into 16 newborn mice of Kunmin strain, the incidence of leukemia was 250% (all were lymphocytic). The results showed that both the cell-free extract and culture supernate from mouse SRS-82 cell line were leukemogenic. The relationship between the virus and the type of leukemia induced remains to be studied.
