The inhibitory effect of 3', 5'-cCMP onT-lymphoblastic cell line JM
- VernacularTitle:胞苷环3′,5′一磷酸对T原淋巴细胞株JM的抑制作用
- Author:
Mingchun WANG
- Publication Type:Journal Article
- Keywords:
Cytidine cyclic monophosphate;
Cell line
- From:
Chinese Journal of Pathophysiology
1986;0(04):-
- CountryChina
- Language:Chinese
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Abstract:
The T-lymphoblastic JM cell line was used to study the influence of the 3′, 5′-cCMP on cell growth. S-Phase parasynchronization was induced by incubation with 0.2mM thymidine for 20 hrs. M-phase parasynchronization was induced by a two step method: Incubation with thymidine was performed first as described above. 11 hours after cell release, colcemide 0.1?g/ml was added to act for 6 hrs. Incubatiou with 3′,5′-cCMP was performed at the time of release. Flow cytometry was performed by an Ortho Cytofluograph System 30.3′, 5′-cCMP can inhibit cell growth during the period from S-phase to G_2/M-phase aud from G_1-phase to S-pase as well. In contrast, 5-CMP, 2′,3′-cCMP and 5-dCMP have no inhibiting effect on cell growth, but can stimulate cell growth in S-phase. Inhibiting effect of 3′,5′-cCMP on cell growth was also present in asynchronous cell cultures.
