Studies on acute incomplete cerebral ischemic and reperfusion damages in rabbits
- VernacularTitle:家兔急性不完全性脑缺血及重灌流的实验研究
- Author:
Yingying LI
- Publication Type:Journal Article
- Keywords:
Cerebral ischemia;
Perfusion
- From:
Chinese Journal of Pathophysiology
1986;0(02):-
- CountryChina
- Language:Chinese
-
Abstract:
Severe incomplete cerebral ischemia was induced in 70 rabbits using bilateral carotid artery occIusion coupled with hemorragic hypotension. 60 minutes of ischemia was followed by 2 hours of reperfusion. The electroencephalographic activity, cardiac output and mean artery blood pressure were measured at regular intervals. The activities of lactic dehydrogenase(LDH) and creatine phosphokinase(CPK) in cerebral venous blood were measured before and after the insult. The contents of water, sodium, potassium and cyclic uncleotides were determined in cerebral cortex after 60 min of ischemia and 120 min of reperfusion respectively. The histological and ultrastructural alterations were observed. The results indicate that the acute incomplete cerebral ischemie model in the rabbit can be successfully established by hypotension plus hypoperfusion. Its characteristics are severe suppression of electroencephalographic activity, significant eIevation of LDH and CPK, an increase in the contents of water and sodium. Ischemic edema was demonstrated morphologically in cerebral cortex. During the reperfusion peroid, initially some recovery of the electroencephaiographic activity appeared but then severe suppression occurred; the severety of brain edema was exacerbated; the content of cAMP was increased further and the activities of LDH and CPK were still significantly elevated. Severe ischemic tissue damage in cerebral cortex were morphologically demonstrated, of which the ultrastructural alterations were more remarkable. These results suggested that the ischemic tissue damage might be aggravated on reperfusion. The possible mechanisms were discussed. The cerebral circulatory disturbance and parenchymal insult during reperfusion peroid might play an important role in developing the irreversible postischemic brain damage.
