Determination of glucocorticoid receptors in canine peripheral leukocytes
- VernacularTitle:狗外周血白细胞糖皮质激素受体的测定
- Author:
Zonghai HUANG
- Publication Type:Journal Article
- Keywords:
Receptors, glucocorticoid;
Radioligand assay;
Leukocytes;
Dogs
- From:
Chinese Journal of Pathophysiology
1986;0(02):-
- CountryChina
- Language:Chinese
-
Abstract:
We have established a method for the measurement of glucocorticoid receptors (GCR) in canine peripheral leukocytes using the radio-ligand binding assay with (~3H) dexamethasone (Dex) as the ligand. The Scatchard plot analysis of (~3H) Dex specific binding to leukocytes showed a single class of binding sites. The apparent view of binding capacity (Ro) and the equilibrium dissociation constant (Kd) were 7.30 ? 1.98 fmol/10~6 cells and 4.69 ? 2.65nM (x?SD, N = 5) respectively. The saturable binding sites showed a high steroid specificity. The specific binding sites in peripheral leukocytes from 22 healthy adult mongrel dogs in normal unstressed condition have been measured by the single saturated analysis with (~3H) Dex at a concentration of 10 nM. The results showed that the number of (~3H)Dex specific binding sites was 3745?486 sites/cell (x?SD). There was no significant difference between male and female dogs in GCR levels. The authors believe that as it is easy to obtain test materials, the result of reduplication is satisfactory, and blood consump tion is low, it is a method suitable for the general laboratory.
