Establishment and characterization of SAC clone system from a mouse SRS ascitic tumour
- VernacularTitle:小鼠SRS腹水瘤SAC克隆系列的建立及其鉴定
- Author:
Han XU
- Publication Type:Journal Article
- Keywords:
Clone cells
- From:
Chinese Journal of Pathophysiology
1986;0(02):-
- CountryChina
- Language:Chinese
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Abstract:
A clone system designated SAC was established from a SRS-82 mouse ascitic turnout cell line by using limited dilution method. Clones SAC-ⅡB_2, SAC-ⅡB_3 of this system were found to be different in their morphology, growth pattern, karyotypes and tumorigenicity from their parent cell line. Both the two clones and their parent cell line were X-C assay positive, and type A and type C virus particles were found in the cytoplasm and cell surface of these cells electronmicroscopically. Cell surface markers(ALS, Thy, SmIg G) of these two clones and their parent cell line were investigated by ABC immunohistochemical method and immunofluorescent staining technique, the results of which indicate that all of them belong to T cell origin.
