Protective effect of extracorporeal membrane oxygenation on Maastricht type Ⅱ donation after cardiac death liver transplantation in pigs
10.3760/cma.j.issn.0254-1785.2016.11.009
- VernacularTitle:体外膜肺氧合在猪模拟马斯特里赫特Ⅱ类心脏死亡供肝肝移植中的应用
- Author:
Lei LIU
;
Ning MA
;
Yuan SHI
;
Yan LIU
;
Jing CHEN
;
Sinan GAO
;
Jianfeng FU
;
Minghao SUI
;
Zhongyang SHEN
- Keywords:
Pigs;
Extracorporeal membrane oxygenation;
Maastricht type Ⅱ;
Cardiac death;
Liver transplantation
- From:
Chinese Journal of Organ Transplantation
2016;37(11):682-685
- CountryChina
- Language:Chinese
-
Abstract:
Objective To detect the protective effect of extracorporeal membrane oxygenation (ECMO) on Maastricht type Ⅱ donation after cardiac death (DCD) liver transplantation in pigs.Methods Twenty mini-pigs were randomized into ECMO group (n =10) and control group (n =10).Then 10 pigs in each group were randomized into donors and recipients.Maastricht type Ⅱ DCD model was induced in all of the 10 donors.Donors of ECMO group received 2-h ECMO after cardiac death,then underwent liver graft procurement.The donors of control group underwent liver procurement directly after cardiac death.Recipients of two groups underwent orthotopic liver transplantation without venovenous bypass.During this procedure,vital signs were monitored continuously,lactate and liver biochemistry were tested,and 5-day survival rate was observed.Results Maastricht type Ⅱ DCD model was successfully built in all of the donors with consequent dark liver.For donors of ECMO group,liver turned sanguinous and soft quickly after treatment of ECMO.There were no significant differences in operation time,anhepatic time and anhepatic hemodynamic changes between these two groups (P ＞ 0.05).As compared with control group,ECMO group had better hemodynamic parameters 30 min after reperfusion,lower lactate,ALT and AST levels 30 min after reperfusion and before closing the abdomen,and higher 5-day survival rate (P ＜ 0.05).Conclusion ECMO may improve the quality of Maastricht type Ⅱ DCD liver graft,and increase the survival rate of DCD liver transplantation.
