Analysis of mutations detection in 23S rRNA gene locus of Mycoplasma pneumoniae among children
10.3969/j.issn.1673-4130.2017.06.014
- VernacularTitle:儿童肺炎支原体23SrRNA基因位点突变检测分析
- Author:
Fen PAN
;
Leijun MENG
;
Huihong QIN
;
Tiandong ZHANG
;
Hong ZHANG
- Keywords:
Mycoplasma pneumoniae;
23S rRNA;
drug-resistance gene;
clinical data
- From:
International Journal of Laboratory Medicine
2017;38(6):760-762
- CountryChina
- Language:Chinese
-
Abstract:
Objective To understand the mutations of macrolide resistance gene locus (23S rRNA) of Mycoplasma pneumoniae (MP) and its correlation with clinical features .Methods A total of 354 respiratory tract samples were collected from children pa-tients with pneumonia .MP and its mutations in 23S rRNA gene locus were detected by real-time PCR .The children cases of MP positive were divided into the mutation group and non-mutation group .Then the clinical data were compared between the two groups .Results Among 354 respiratory tract samples ,166 cases(46 .9% ) were MP positive ,moreover the mutation of 23S rRNA gene locus existed in 135 MP positive samples with the positive detection rate of 81 .3% ,while no 23S rRNA gene locus mutations were detected in 31 samples .Analyzing the clinical data of the mutation group and non-mutation group found that there was no sta-tistical difference in the aspects of age and gender between the two groups .The occurrence rates of severe pneumonia and extrapul-monary complications in the mutation group were higher than those in the non-mutation group (P<0 .05) ,moreover the average hospitalization time and fever duration in the mutation group were longer than those in the non-mutation group (P<0 .05) .Conclu-sion 23S rRNA gene locus mutation has higher detection rate ,prompting that MP shows high resistant rate to macrolides ,which could provide a certain basis for treatment of M P infections .
