Study on the Spectrum-effect Relationship of Anti-inflammation Effect in vitro of Extracts from Uygur Medicine Hyssopus cuspidatus
10.6039/j.issn.1001-0408.2017.10.18
- VernacularTitle:维药神香草提取物体外抗炎作用的谱效关系研究
- Author:
Yan MAO
;
Jinhua HE
;
Xiaocui CAI
;
Cai TIE
;
Xintang WANG
- Keywords:
Hyssopus cuspidatus;
Extracts with different polarities;
Anti-inflammation activity;
Fingerprint;
Spectrum-effect re-lationship;
Inflammatory factor
- From:
China Pharmacy
2017;28(10):1364-1367
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE:To provide reference for clarifying the pharmacodynamic material basis of anti-inflammation effect of Uygur medicine Hyssopus cuspidatus. METHODS:HPLC was conducted to establish the fingerprint of 8 extracts with different po-larities. Using macrophage RAW264.7 as object,the inhibitory effect of extracts with different polarities on inflammatory factor ni-tric oxide(NO),TNF-α and IL-6 in supernatant of cell culture medium was detected,the differences of anti-inflammatory activity in vitro were compared. Grey correlation analysis method was used to analyze the spectrum-effect relationship of peak area of common peaks and anti-inflammation activity. RESULTS:The fingerprint of 8 extracts with different polarities showed obvious differences. Anti-inflammation in vitro results suggested that 30% ethanol extract had the strongest inhibitory effect on inflammatory cytokines in vitro. There were 14 common peaks in the established HPLC fingerprints,the 5 common peaks that was closely related to the in-hibitory effect of inflammatory factors in vitro were peak 8,6,5,10,13,respectively;peak 8 was rosmarinic acid. CONCLUSIONS:The established fingerprint and its spectrum-effect relationship with anti-inflammation activity in vitro can provide certain reference for its pharmacodynanic material basis study.
