Analysis of the volatile oil in fructus lycii(Lycium barbarum L.) by GC-MS and HELP method
10.3760/cma.j.issn.1673-4246.2017.04.013
- VernacularTitle:气质联用技术结合直观推导式演进特征投影法分析枸杞子挥发油成分
- Author:
Boqian LI
;
Jiao XU
;
Jianmei GU
;
Rui YANG
;
Lijuan CHEN
- Keywords:
Fructus lycii;
Volatile oils;
Gas chromatography-mass spectrometry;
Heuristic evolving latent projections;
Chemical qualitative analysis
- From:
International Journal of Traditional Chinese Medicine
2017;39(4):347-350
- CountryChina
- Language:Chinese
-
Abstract:
Objective Gas chromatography-mass spectrometer (GC-MS) technology combined with Heuristic evolving latent projections (HELP) method were used to qualitatively analyze the volatile oil of fructus lycii (Lycium barbarumL.)Methods With the best temperature-programmed chromatographic condition, overlapping peaks among the total ion chromatogram were separated. Then an automatic mass spectral deconvolution and identification system (AMDIS) was used to identify volatile components comprehensively. Finally, the pure chromatographic peaks were matched with NIST 05a mass spectra library for giving the precise qualitative result. Results Totally, 29 compounds in fructus lycii (Lycium barbarum L.) were identified accurately.Conclusions Compared with the traditional qualitative analysis method, the new established method which GC-MS technology combined with HELP could get tremendous advantages. It not merely enhanced the accuracy of identification but also solved the bottleneck of handling the inseverable compounds.
