Effects of bioactive glass and extracted dentin proteins on human dental pulp cells
10.3969/j.issn.1671-167X.2017.02.026
- VernacularTitle:生物活性玻璃和牙本质浸提蛋白对人牙髓细胞的作用
- Author:
Yi XIN
;
Sainan WANG
;
Caiyun CUI
;
Yanmei DONG
- Keywords:
Bioactive glass;
Extracted dentin proteins;
Odontogenic differentiation;
Tooth reminera-lization
- From:
Journal of Peking University(Health Sciences)
2017;49(2):331-336
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To investigate the proliferation,odontogenic differentiation and mineralization of human dental pulp cells (HDPCs) on bioactive glass(BG) and extracted dentin proteins(EDP).Me-thods: Primary HDPCs were isolated from third molars by enzyme digestion and were cultured in Dulbecco's minimum essential medium (DMEM).Then the 4th generation of HDPCs was cultured with DMEM,which contained BG-EDP,BG,and EDP,respectively.Meanwhile HDPCs were cultured in DMEM as control group.Proliferation of HDPCs was evaluated by 3-(4,5-dimethyl-2-thiazolyl)-2,5-diphenyl-2-H-tetrazolium bromide(MTT) colorimetric assay.Odontogenic differentiation was determined by alkaline phosphatase (ALP) activity assay and real-time PCR.Mineralization was investigated by Alizarin red staining and cetylpyridinium chloride (CPC) assay.Results: The proliferation of HDPCs was increased significantly in BG-EDP group on 3,7,and 9 d(optical density value:1.36±0.06,2.52±0.20,2.72±0.29) compared with BG(optical density value: 1.20±0.26,2.33±0.26,2.50±0.30),EDP(optical density value: 1.13±0.15,2.10±0.13,2.38±0.22) and control group(optical density va-lue: 0.84±0.17,1.84±0.18,1.95±0.19),P<0.05.After 7 days,ALP activity of BG-EDP group had no statistical difference compared with EDP group and control group;the expression of odontogenic differentiation genes (DSPP,DMP-1) showed no difference among all the groups(P>0.05).After 14 days,ALP activity of BG-EDP group (56.67±1.83) was significantly upregulated compared with EDP group (41.98±9.71) and control group (30.82±6.70),P<0.05,but had no statistical difference compared with BG group (56.29±6.20),P>0.05;DSPP gene expression was upregulated significantly in BG-EDP group (5.79±1.94) compared with the other groups (P<0.05);DMP-1 gene expression of BG-EDP group (3.87±1.87) increased but had no statistical difference compared with the other groups (P>0.05).The alizarin red staining showed more mineral nodules in BG-EDP group,the cetylpyridinium chloride semi-quantification presented higher calcification in BG-EDP group (0.27±0.01) compared with the other groups (P<0.05).Conclusion: Compared with either BG or EDP,BG-EDP significantly promotes the proliferation,odontogenic differentiation and mineralization of HDPCs.
