Effects of Jinmaitong serumon the expression of β-catenin, GSK-3β and myelin protein zeroin Schwann cells of rats cultured in high glucose medium
- VernacularTitle:筋脉通含药血清对高糖培养大鼠雪旺细胞β-catenin、GSK-3β及髓鞘零蛋白表达的影响
- Author:
Ying SUN
;
Xiaochun LIANG
;
Wei LIU
;
Di LIU
- Keywords:
Jinmaitong;
Schwann cells;
Wnt/β-catenin;
myelin protein zero
- From:
Basic & Clinical Medicine
2017;37(4):484-487
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the effect of serum containing different concentrations of traditional Chinese medicine Jinmaitong on β-catenin, GSK-3β and P0 in Schwann cells cultured in high glucose medium.MethodsTwenty-five male Sprague-Dawley rats were randomly treated with 5, 10, 15 and 20 times of Jinmaitong and distilled water.Schwann cells were divided into six groups, which are control group, high glucose group, 5 times group, 10 times group, 15 times group, 20 times group.72 hours later, the proliferative activity of SCs cells were detected by CCK, the mRNA and protein expression of β-catenin, P0 and GSK-3β was detectived by rt-PCR and Western blot.Results High glucose medium could inhibit the proliferation of Schwann cells, down-regulate the expression of β-catenin and P0(P<0.01), and up-regulate the expression of GSK-3β(P<0.05) mRNA significantly.But Jinmaitong can invert the results (P<0.01, P<0.05).Conclusions High glucose medium will injure the proliferation of Schwann cells, but Jinmaitongcan increase the proliferation activity of Schwann cells, and promotes the secretion of P0 partially dependent on up-regulating the activity of β-catenin and down-regulating the activity of GSK-3β.
