Effect of PSMA7 on RB pathway in A549 cells
10.3969/j.issn.1000-484X.2017.04.008
- VernacularTitle:PSMA7对A549细胞中RB途径的影响
- Author:
Xiang HUANG
;
Yuheng ZHONG
;
Jiayu TAN
;
Xuewei XU
;
Rui LIANG
;
Shaoxia LIANG
;
Jing ZHAO
- Keywords:
HSPC238;
RB pathway;
Cell cycle;
Western blot
- From:
Chinese Journal of Immunology
2017;33(4):516-519
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To investigate the effect of upregulated and downregulated PSMA7 on the cell cycle and Cyclin D1,CDK4,P16,Rb of RB pathway in A549 cells.Methods:Transfected upregulated pcDNA3.1-PSMA7 vecter and downregulated pGPU6/Hygro-PSMA7-265 vecter into A549 cells,and then tested the effect of PSMA7 on the cell cycle of A549 cells by flow cytometry,and detected the protein level of Cyclin D1,CDK4,P16,Rb by Western blot.Results:Compared with the control group,the cell cycle of the A549 cells did not change significantly,and the expression of Cyclin D1,CDK4 decreased but P16,Rb increased when PSMA7 was upregulated.Compared with the control group,the proportion of phase G0/G1,G2/M of the A549 cells decreased and phase S increased,and the expression of Cyclin D1,CDK4 increased but P16,Rb decreased when PSMA7 was downregulated.There was statistical significance for those results.Conclusion:PSMA7 could affect the expression of Cyclin D1,CDK4,P16,Rb protein level of RB pathway in A549 and promoted the A549 cells into phase S when it′s downregulated.
