Experimental study of tumor-specific CTL induced by rmIL-18 treated on hepatocellular carcinoma
10.3969/j.issn.1000-484X.2017.04.014
- VernacularTitle:应用rmIL-18诱导肿瘤特异性CTL治疗肝癌的实验研究
- Author:
Xuguang MI
;
Lei LIU
;
Shouqing LI
;
Haifeng WEI
;
Shufen XU
;
Yan TAN
;
Yanqiu FANG
- Keywords:
CCs;
rmIL-18;
CTL;
Anti-tumor
- From:
Chinese Journal of Immunology
2017;33(4):545-548
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To research rmIL-18 in vitro culture system CCs induce tumor-specific cytotoxic T lymphocytes CTL and anti-tumor effect in mice.Methods:Used Stem SepTM immune magnetic cells separation method to culture mouse spleen NK cells,T cells and DCs,established culture systems in vitro;used of different approaches,different doses rmIL-18 to immunize HCC tumor-bearing mice,researched the effect of rmIL-18 on tumor growth rate and survival time.Results:rmIL-18 could induce and promote tumor-specific CTL-mediated killing effects in vitro culture system;tumor-specific CTL could significantly inhibit tumor growth(P<0.01) of and prolong the survival time of liver cancer tumor-bearing mice(P<0.01),and the effect was increased with rmIL-18 concentration increased(P<0.01),and intratumoral injection was superior to intraperitoneal injection(P<0.01).Conclusion:rmIL-18 can induce tumor-specific CTL in vitro and play a role in anti-liver cancer in mice.
