Effects of RhoGDI2 gene silencing by siRNA on proliferation and invasion of colon carcinoma cell lines
10.3969/j.issn.1000-484X.2017.04.015
- VernacularTitle:小分子干扰RNA沉默RhoGDI2基因表达对结肠癌细胞增殖侵袭的影响及其机制
- Author:
Wenrui WANG
;
Hongli YAN
;
Lijun MA
;
Siqi LIU
;
Zhenjing JIN
- Keywords:
Small interfering RNA;
RhoGDI2;
Colon cancer;
Epithelial-mesenchymal transition
- From:
Chinese Journal of Immunology
2017;33(4):549-553
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To study the silencing gene expression level of RhoGDI2 small interfering RNA(siRNA)and the colorectal cancer cell malignant behaviors such as cell proliferation,migration,invasion.Methods:Testing RhoGDI2 expression using Westen blot analysis and Real-time reverse transcription polymerase chain reaction(RT-qPCR)in the colorectal cancer cell lines of RKO,HT29,SW620,SW480,HCT116.The siRNA of RhoGDI2 with Lipofecta mineTM2000 was transfected into target cells,as well as negative control and normal control groups.Cell counting kits(CCK-8)to detect proliferation,Wound healing assay and the Transwell plate migration and invasion was detected.The epithelial-mesenchymal transition(EMT)relevant protein E-cadherin/Vimentin expression was detected.Results:Human colon cancer cell lines RhoGDI2 expression levels decreased in the order of RKO,HT29,SW620,SW480,HCT116:siRNA inhibited RhoGDI2 expression rate of RKO cell by 70%;in silence group,negative control group and blank contro1 group,the proliferation rates were(0.683±0.013),(0.866±0.088),(0.905±0.008),P<0.05;Wound healing assay and Transwell assay suggested RhoGDI2 silencing could inhibit migration;siRNA interference of colon cancer cells downregulated Vimentin,but upregulated E-Cadherin protein.Conclusion:RhoGDI2 down-regulation could significantly inhibit the cell proliferation,migration,invasion of colon cancer cell.
