IL-10 promotes ocular neovascularization by regulating macrophages
10.3969/j.issn.1674-8115.2017.03.002
- VernacularTitle:白细胞介素-10调控巨噬细胞以促进眼底新生血管形成
- Author:
Ailing SUI
;
Ting SU
;
Yushuo GAO
;
Yanji ZHU
;
Bing XIE
- Keywords:
interleukin-10;
macrophage;
hypoxia-inducible factor-1α;
vascular endothelial growth factor
- From:
Journal of Shanghai Jiaotong University(Medical Science)
2017;37(3):278-287
- CountryChina
- Language:Chinese
-
Abstract:
Objective · To investigate the role of interleukin-10 (IL-10) in regulating ocular neovascularization (NV). Methods · Expression of IL-10 was investigated in mice with oxygen-induced retinopathy (OIR) and transgenic mice with VEGF expression in photoreceptors by immunofluorescence,RT-PCR, and Western blotting. Mice deficient in IL-10 were used to test the effect of IL-10 in retinal, sub-retinal, and choroidal NV. Results · In OIR mice and transgenic mice with VEGF expression in photoreceptors, the staining intensity and mRNA expression of IL-10 were increased. Mice deficient in IL-10 showed a significant reduction in ischemia-induced retinal NV, and choroidal NV at rupture sites in Bruch's membrane. Mice lacking IL-10 showed reduced levels of hypoxia-inducible factor-1α (HIF-1α) and suppression of ischemia-induced expression of VEGF and VEGF receptor 1. Macrophage was regulated and reduced in ischemic retina of mice with IL-10 deficiency. Conclusion · IL-10 stimulates ocular NV through modulation of HIF-1α and its target genes VEGF and VEGF receptor 1. IL-10 promotes ocular NV via macrophage response to retina ischemia.
