The characteristics of the full-field flash electroretinogram in rats with oxygen induced retinopathy
10.3760/cma.j.issn.1005-1015.2017.02.017
- VernacularTitle:氧诱导视网膜病变大鼠模型的全视野闪光视网膜电图特征
- Author:
Li XIE
;
Yi LUO
;
Ping WANG
;
Lan MA
;
Na LI
;
Linhui XIE
- Keywords:
Retinal diseases/physiopathology;
Electroretinography;
Animal experimentation
- From:
Chinese Journal of Ocular Fundus Diseases
2017;33(2):186-189
- CountryChina
- Language:Chinese
-
Abstract:
Objective To observe the characteristics of the full-field flash electroretinogram (F-ERG) in rats with oxygen induced retinopathy (OIR).Methods Twenty-four neonatal Sprague Dawley rats were divided into OIR group and control group.In OIR group,12 rats were exposed to (75 ± 2)％ oxygen for 7 days and then to room air for 7 days;in control group,12 rats were raised in room air for 14 days.At postnatal day 21,F-ERG tests were performed to examine the rod response,the maximum mixing reaction and the cone reaction.Results Compared with the control group,the b-wave amplitudes decreased (t=3.650) and the implicit times increased (t=2.410) in rod response in OIR group,the differences were statistically significant (P＜ 0.05);the a-and b-wave amplitudes decreased (t=3.333,2.562) and the implicit times increased (t=2.725,2.482) in the maximum mixing reaction in OIR group,the differences were statistically significant (P＜0.05).There was no difference between OIR and control group on a-and b-wave amplitudes (t=0.650,0.204) and implicit times (t=0.422,0.076) in cone response (P＞0.05).0.001 cd.s/m2 light intensity stimulation on rats F-ERG wave almost no response.0.010 cd.s/m2 light intensity stimulation on rats can be recorded to the rod response waveform,with the increase of light intensity,the amplitude of b-wave increases,the a-wave extraction.Conclusions F-ERG of OIR rat showed that the amplitude and sensitivity of the rod response and maximal rodcone response was decreased.The intensity of light had effect on the OIR rod cells,and the amplitude of b-wave increased with the increase of light intensity,the a-wave extraction.
