Tricin's inhibitory effects on TLR4/MyD88/NF-κB pathway of alveolar macrophages in asthma mice
10.3969/j.issn.1001-1528.2017.03.002
- VernacularTitle:苜蓿素对哮喘小鼠肺泡巨噬细胞TLR4/MyD88/NF-κB通路的抑制作用
- Author:
Yuting YIN
;
Lili LIU
;
Liyan LIU
- Keywords:
tricin;
asthma;
airway inflammation;
anti-inflammation;
alveolar macrophages;
TLR4/MyD88/ NF-κB pathway
- From:
Chinese Traditional Patent Medicine
2017;39(3):450-454
- CountryChina
- Language:Chinese
-
Abstract:
AIM To observe the inhibitory effects of tricin on the airway inflammation in low concentration LPS induced asthma mice.METHODS The model of asthma mice with airway inflammation was induced by low concentration LPS plus OVA.There were four groups:blank control group,asthma model group,tricin treatment group and dexamethasone (positive drug) treatment group.The levels of inflammatory factors,including NO,TNF-α and IL-1β,in bronchoalveolar lavage fluid (BALF) were detected by ELISA;the pathology of lung tissue was observed by HE staining;the mRNA and protein expressions of TLR4,MyD88 and NF-κB p65 in alveolar macrophages were detected using real-time quantitative PCR (RT-PCR) and Western blot.RESULTS Tricin could significantly decrease the levels of NO,TNF-α and IL-1β in BALF of asthma mice and improve lung inflammation.It could also down-regulate the relative mRNA and protein expressions of TLR4,MyD88 and NF-κB p65.CONCLUSION Tricin has inhibitory effects on airway inflammation in asthma mice,whose mechanism may be related to the intervention of TLR4/MyD88/NF-κB pathway.
