Genetic analysis of Echinococcus granulosus strain in Liaoning Province, China
10.3969/j.issn.1002-2694.2017.02.004
- VernacularTitle:辽宁省朝阳市细粒棘球病的基因型分析
- Author:
Xiaoli WANG
;
Fei LI
;
Jinhui LI
;
Jing LIU
;
Yacai LI
;
Yang BAI
;
Chunli AN
- Keywords:
Echinococcus granulosus;
Cox1 gene;
genotype;
molecular epidemiology
- From:
Chinese Journal of Zoonoses
2017;33(2):110-114
- CountryChina
- Language:Chinese
-
Abstract:
To analyze the genotype of Echinococcus granulosus found in Liaoning Province,hydatid cyst was taken from the patient's liver,DNA of cyst was isolated and mitochondrial cox1 gene was sequenced substantially.Sequence results was analyzed by Blast and compared with similar strains in GenBank.Results showed that molecular analysis confirmed the echinococcosis by identical sequence of our strain with E.granulosus (AF297617) G1 genotype,with 0.1％ difference.And the sequence of our strain was consistent with 25 other strains in different countries.In conclusion,genotyping of mitochondrial gene coxl is a very useful tool for diagnosis of echinococcosis.We first reported a strain of E.granulosus in Liaoning Province,which provide novel molecular epidemiological data of this zoonosis.
