Application of native hapten-gel diffusion test in differential diagnosis of Brucella-infected antibody and S2 or Rev.1-vaccinated antibody
10.3969/j.issn.1002-2694.2017.02.007
- VernacularTitle:半抗原-琼脂扩散试验鉴别布鲁氏菌感染抗体和S2、Rev.1免疫抗体的研究与应用
- Author:
Zhen YANG
;
Bo NI
;
Lili TIAN
;
Weixing FAN
- Keywords:
brucellosis;
NH-GD;
differential diagnosis
- From:
Chinese Journal of Zoonoses
2017;33(2):126-130
- CountryChina
- Language:Chinese
-
Abstract:
The aim of the present study was to work on the efficiency of differential diagnosis of native hapten-gel diffusion assay (NH-GD) on the background of vaccination with S2 or Rev.1.The conditions of NH-GD assay was firstly optimized,its sensitivity,specificity,repeatability and ability of differential diagnosis were determined respectively,and its test result was compared with that of fluorescence polarization assay (FPA).The results showed NH-GD assay with good specificity and repeatability could differentiate Brucella-infected antibody from vaccinated antibody after vaccination with S2 or 122 days after vaccination with Rev.1.And the result of NH-GD assay was highly consistent with that of FPA,which was simple to operate and needed a few simple equipment.Therefore,NH-GD assay was a good method for sheep brucellosis surveillance in China and especially suitable for application in grass-roots areas.
