Effect of interferon-γ and exogenous indole on the growth of Chlamydia trachomatis
10.3969/j.issn.1002-2694.2017.02.008
- VernacularTitle:γ-干扰素及外源性吲哚对泌尿生殖道沙眼衣原体生长影响的探讨
- Author:
Zhichao LIU
;
Yuanjun LIU
;
Jiangang LIU
;
Quanzhong LIU
- Keywords:
Chlamydia trachomatis;
Hela cell;
interferon-γ;
exogenous indole
- From:
Chinese Journal of Zoonoses
2017;33(2):131-135
- CountryChina
- Language:Chinese
-
Abstract:
We investigated the effects of γ-interferon and exogenous indole on the growth of domestic dominant standard strains and clinical straìns of Chlamydia trachomatis E-UW-5/Cx,and compared with the dominant strains of D-UW-5/Cx abroad.We used DMEM-10,DMEM-10 containing 5 ng/mL recombinant human interferon gamma (referred to as DMEM-10+IFN) and DMEM-10 containing 5 ng/mL recombinant human interferon gamma and 50 μM exogenous indole (referred to as DMEM-10+IFN+IND) to culture C.trachomatis,and then we fixed it with methanol to count inclusions after 48 hours,observing the influence of r-interferon and exogenous indole on the growth of C.trachomatis standard strains(E,D) and clinical strains.Results showed that the count of Chlamydia inclusion bodies in DMEM-10+IFN group was significantly lower than others (P＜0.05);no significant difference was found (P＞0.05) between the count of DMEM-10 group between DMEM-10+IFN+IND group.There were no significant difference between the E and D standard or clinical strains (P＞0.05).Under the effect of IFN-γ,the growth of domestic dominant strain E-UW-5/Cx C.trachomatis was significantly inhibited.After adding exogenous indole,C.trachomatis can escape the scavenging activity of IFN-γγto restore the infection vitality.
