Anti-inflammatory and analgesic effects in vivo and bacteriostasis effect in vitro of diuretic mixture
10.7501/j.issn.1674-6376.2017.02.011
- VernacularTitle:利尿合剂抗炎镇痛及体外抑菌作用研究
- Author:
Wenxian GUO
;
Weibo DAI
;
Quanxi MEI
;
Hongnian LI
- Keywords:
diuretic mixture;
analgesic and anti-inflammation;
bacteriostasis effect in vitro;
ear swelling;
twisting reaction;
capillary permeability
- From:
Drug Evaluation Research
2017;40(2):201-205
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the analgesic and anti-inflammatory effects of diuretic mixture and its bacteriostasis effect in vitro,and to provide scientific basis for clinical application.Methods Ear swelling test induced by xylene,twisting reaction test induced by acetic acid,capillary permeability increase in abdominal cavity of mice induced by acetic acid,and pain test induced by formalin were used to observe the analgesic and anti-inflammatory effects of diuretic mixture at high,middle and low doses (crude drug 18.0,9.0,and 4.5 g/kg).Bacteriostatic activities of diuretic mixture were tested by K-B paper disc diffusion method.Results Diuretic mixture alleviated ear edema in mouse model at high dose (P ＜ 0.01).Diuretic mixture at high,middle,and low dose could effectively decrease the twisting reaction (P ＜ 0.01),inhibit capillary permeability (P ＜ 0.05 or 0.01),and ease the ache degree of mice induced by formalin in the first phase (P ＜ 0.01),but there was no significant difference in the degree of pain intensity of phase Ⅱ.The inhibitory rates of diuretic mixture on Staphylococcus aureus and Escherichia coli were 95.04％ and 37.44％,respectively.Conclusion Diuretic mixture has significant effects on analgesia and anti-inflammation and against S.aureus and E.coli in vitro.
