Preparation of TPGS-modified artesunate liposomes and their in vitro anti-tumor activity
10.3969/j.issn.1001-1528.2017.03.010
- VernacularTitle:TPGS修饰青蒿琥酯脂质体的制备及其体外抗肿瘤活性
- Author:
Cheng HU
;
Kun LIANG
;
Rui AN
;
Xinhong WANG
;
Lisha YOU
- Keywords:
artesunate;
liposomes;
D-α-tocopherol polyethylene glycol 1000 succinate (TPGS);
preparation;
in vitro anti-tumor activity;
human hepatoma HepG2 cells
- From:
Chinese Traditional Patent Medicine
2017;39(3):492-498
- CountryChina
- Language:Chinese
-
Abstract:
AIM To prepare D-α-tocopherol polyethylene glycol 1000 succinate (TPGS)-modified artesunate liposomes and to investigate the in vitro anti-tumor activity.METHODS The liposomes prepared by thin-film dispersion method were characterized by transmission electron microscopy and particle size analyzer,and the encapsulation efficiency was determined by ultrafiltration centrifugation.The liposomes' cytotoxicity to human hepatoma HepG2 cells was evaluated by MTT method.RESULTS The average particle size,PDI,Zeta potential,encapsulation efficiency,drug loading of the liposomes were 126.7 nm,0.182,-10.1 mV,78.8％ and 18.38％,respectively.The liposomes displayed a significant inhibition on HepG2 cells with the IC50 value of 0.034 μmol/mL.CONCLUSION Compared with non-TPGS-modified artesunate liposomes,the TPGS-modified artesunate liposomes prepared by this method afford smaller vesicle size,better stability and higher encapsulation efficiency with stronger in vitro anti-tumor activity.
