Determination of six catechins in Xinnaojian Capsules (Tablets) by QAMS
10.3969/j.issn.1001-1528.2017.03.016
- VernacularTitle:一测多评法测定心脑健胶囊(片)中6种儿茶素
- Author:
Jing XIE
;
Jie WEI
;
Luwei ZHOU
;
Jinlian HE
;
Leilei DU
- Keywords:
Xinnaojian Capsules (Tablets);
epigallocatechin;
catechin;
epicatechin;
epigallocatechin gallate;
gallocatechin gallate;
epicatechin gallate;
quantitative analysis of multi-components by single marker (QAMS)
- From:
Chinese Traditional Patent Medicine
2017;39(3):523-527
- CountryChina
- Language:Chinese
-
Abstract:
AIM To establish a quantitative analysis of multi-components by single marker(QAMS) method for the content determination of six catechins in Xinnaojian Capsules (Tablets) (tea extract).METHODS The analysis of 50％ methanol extract of this drug was performed on a 35 ℃ thermostatic Shimadzu Wonda Cract ODS-2 column (4.6 mm ×250 mm,5 μm),with the mobile phase comprising of 0.5％ acetic acid (A)-acetonitrile (B) flowing at 1.0 mL/min in a gradient elution manner,and the detection wavelength was set at 280 nm.With epigallocatechin gallate as an internal standard,the relative correction factors of epigallocatechin,catechin,epicatechin,gallocatechin gallate and epicatechin gallate were calculated,from which the content determination was made.RESULTS Six constituents showed good linear relationships within their own ranges (r ≥ 0.999 8),whose average recoveries were 96.00％-98.47％ with the RSDs of 2.09％-2.91％.The results obtained by QAMS method approximated those obtained by external standard method.CONCLUSION This simple and reliable method can be used for the quality control of Xinnaojian Capsules (Tablets).
