Simultaneous determination of four constituents in Xiaojie'an Capsules by HPLC

VernacularTitle:HPLC法同时测定消结安胶囊中4种成分
Author: Gang BAO ; Yamin ZUO ; Jianjun HUANG ; Tian ZHOU ; Wei PAN ; Qing WU ; Xing LI
Keywords: Xiaojie'an Capsules; berberine hydrochloride; palmatine chloride; phillyrin; rutin; HPLC
From: Chinese Traditional Patent Medicine 2017;39(3):536-539
CountryChina
Language:Chinese
Abstract: AIM To establish an HPLC method for the simultaneous content determination of four constituents in Xiaojie'an Capsules (Forsythiae Fructus,Leonuri Herba,Spatholobi Caulis,etc.).METHODS The analysis of chloroform extract of this drug was carried out on a 30 ℃ thermostatic Diamond C1scolumn(250 mm ×4.6 mm,5 μm),with the mobile phase comprising of acetonitrile (A)-0.05 mol/L monosodium phosphate (B) flowing at 1.0 mL/min in a gradient elution manner,and the detection wavelength was set at 278 nm.RESULTS Berberine hydrochloride,palmatine chloride,phillyrin and rutin showed good linear relationships within the ranges of 0.033 7-0.337 2 μg (r =0.999 1),0.054 8-0.548 3 μg (r =0.999 0),0.025 9-0.258 8 μg (r=0.999 2) and 0.008 4-0.084 2 μg (r =0.999 6),whose average recoveries were 98.8％ (RSD =1.3％),99.8％ (RSD =0.7％),98.8％ (RSD =1.3％) and 96.8％ (RSD =1.0％),respectively.CONCLUSION This sensitive and accurate method can be used for the quality control of Xiaojie'an Capsules.