Study on the HPLC Fingerprint of Blumea balsamifera and Its Fake B. riparia
10.6039/j.issn.1001-0408.2017.09.29
- VernacularTitle:艾纳香及其伪品假东风草的HPLC指纹图谱研究
- Author:
Hua FENG
;
Ye YANG
;
Xiangpei WANG
;
Hongmei WU
;
Xianyou YANG
- Keywords:
Blumea balsamifera;
Blumea riparia;
HPLC;
Fingerprint;
Identification
- From:
China Pharmacy
2017;28(9):1257-1261
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE:To establish the HPLC fingerprint for Blumea balsamifera and its fake B. riparia. METHODS:HPLC was performed on the column of Uitimate-C18 with mobile phase of acetonitrile-0.05% phosphoric acid(gradient elution)at a flow rate of 0.6 mL/min,detection wavelength was 270 nm,column temperature was 25 ℃,and injection volume was 7 μL. Using quercetin as a reference,Similarity Evaluation Software for Chromatographic Fingerprint of Traditional Chinese Medicine(2004 A edition)was used for the common peaks identification and similarity analysis of 16 batches of B. balsamifera and 5 batches of B. ri-paria. RESULTS:There were 61 common peaks in the 16 batches of B. balsamifera,similarity degree was 0.931-0.995,which was higher than the similarity degree of 5 batches of B. riparia. CONCLUSIONS:The established fingerprint can provide reference for the identification and quality evaluation of B. balsamifera.
