Comparative study of cholic acid compounds of bezoar on anti-cerebral infarction and regulating endoplasmic reticulum stress
10.7501/j.issn.1674-6376.2017.01.003
- VernacularTitle:牛黄中胆酸类成分改善大鼠脑缺血再灌注损伤及调节内质网应激作用比较
- Author:
Xiaolin XU
;
Chongyang MA
;
Xueqian WANG
;
Guoli WANG
;
Changming ZHAI
;
Wenchao YUE
;
Changxiang LI
;
Xiaoyu ZHANG
;
Xiaodong SHEN
;
Jie MU
;
Qingguo WANG
;
Fafeng CHENG
- Keywords:
endoplasmic reticulin stress;
cerebral ischemia-reperfusion injury;
taurine-conjugated ursodeoxycholic acid;
qingkailing;
taurine;
ursodeoxycholic acid
- From:
Drug Evaluation Research
2017;40(1):11-19
- CountryChina
- Language:Chinese
-
Abstract:
Objective Using middle cerebral artery occlusion (MCAO) model to observe protective effect of effective components of bezoar on brain damage.To discuss the anti-cerebral ischemia mechanism and compare the efficacy of effective components of bezoar from the endoplasmic reticulum stress intervention angle.Methods Rats were stratified randomly divided into sham group,model group,Qingkailing group (positive drug,3 mL/kg),taurine group,ursodeoxycholic acid (UDCA,78 mg/kg) group,taurine-conjugated ursodeoxycholic acid (TUDCA,100 mg/kg) group.Through establish MCAO model in rats,observed the scores of the neurologic impairment,measured infarct volume by TTC.Immunohistochemistry and Western blotting were Used to detect the content of P-PERK,P-EIF2α,and ATF4.Results Compared with sham group,neurologic impairment scores of model group significantly reduced (P ＜ 0.01).Compared with model group,Qingkailing group,UDCA group,and TUDCA group significantly improved neurological function in rats (P ＜ 0.05,0.01).Compared model group,all the treatment groups could significantly reduce the volume of cerebral infarction (P ＜ 0.01).Compared with sham group,expression of P-PERK,P-EIF2α,and ATF4 was significantly increased (P ＜ 0.01).Compared with model group,all the treatment groups reduced the expression of P-PERK,P-EIF2α,and ATF4 in varying degrees,effect of Qingkailing and TUDCA were more obvious.Conclusion The effective components ofbezoar alleviate cerebral ischemia reperfusion injury in rats by inhibiting endoplasmic reticulum stress,the effect of TUDCA is better than that of taurine and UDCA.
