Inhibition on proliferation of hepatoma HepG-2 cell treated with Curcumin combined with Glycyrrhetinic acid
10.7501/j.issn.1674-6376.2017.01.007
- VernacularTitle:姜黄素与甘草次酸联用对肝癌HepG-2细胞增殖的抑制作用
- Author:
Mingxiang CHANG
;
Meimei WU
;
Hangmin LI
- Keywords:
Curcumin,Glycyrrhetinic acid,drug combination,anti-tumor activity;
apoptosis;
cell cycle
- From:
Drug Evaluation Research
2017;40(1):42-47
- CountryChina
- Language:Chinese
-
Abstract:
Objective To study the inhibitory effect of Curcumin combined with Glycyrrhetinic acid on proliferation of HepG-2 cell,and probe the reasonability and scientificity of curcuma combined with glycyrrhiza.Methods The CCK-8 method was used to test the proliferation inhibition rate of HepG-2 cells after treated with Curcumin (10.00,5.00,2.50,and 1.25 μg/mL),Glycyrrhetinic acid (20.0,10.0,5.0,and 2.5 μg/mL) and Curcumin combined with Glycyrrhetinic acid in corresponding concentration for different time (8,16,or 24 h).Flow cytometry was used to detect the cell apoptosis and the cell cycle of HepG-2 cells after treated with Curcumin (5 μg/mL),Glycyrrhetinic acid (10 μg/mL) and Curcumin combined with Glycyrrhetinic acid in corresponding concentration for 24 h.Results Curcumin,Glycyrrhetinic acid and drug combination obviously inhibited the proliferation of HepG-2 cells in a time-and concentration-dependent manner,the effects of combination group was more stronger and showed additive effect or synergistic effect.The apoptosis rate of HepG-2 cells was significantly increased after treated with three groups of drug,combination group showed additive effect;Curcumin,Glycyrrhetinic acid and the drug combination showed significant G2 arrest.Conclusion Curcumin combined with Glycyrrhetinic acid has positive effect on inhabiting the proliferation and promoting apoptosis of HepG-2 cell.Curcuma combined with glycyrrhiza possess reasonability and scientificity.
