Identification of a New Adulterated Dye and Study on Detection Method for 9 Orange-yellow Dyes in Typhae Pollen
10.6039/j.issn.1001-0408.2017.09.20
- VernacularTitle:蒲黄中1种新染色掺假色素的确认及9种橙黄色色素的检测方法研究
- Author:
Zhao GENG
;
Yanfei ZHANG
;
Yan GOU
;
Juan ZHOU
;
Li GUO
;
Qiang WU
- Keywords:
Typhae Pollen;
Dye;
TLC;
LC-MS;
HPLC
- From:
China Pharmacy
2017;28(9):1225-1228
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE:To identify a new adulterated dye,and to establish simultaneous detection method for 9 orange-yel-low dyes in Typhae Pollen. METHODS:TLC and LC-MS were used for the identification of the new adulterated dye;TLC and HPLC method were used for determining 9 orange-yellow dyes. The determination was performed on Welch AQ-C18 column with mobile phase consisting of acetonitrile-0.05 mol/L ammonium acetate solution (gradient elution) at the flow rate of 1.0 mL/min. The detection wavelength were set at 432 nm (lemon yellow,metanil yellow,gold orange O,basic orangeⅡ) and 484 nm (sun-set yellow,acid orange Ⅰ,gold orangeⅡ,orange G,basic orange 21). The column temperature was 35 ℃.The sample size was 10 μL. RESULTS:TLC spots of auramine O,lemon yellow and new adulterated dye were clear and well separated without in-terference from negative control;new adulterated dye was identified as metanil yellow. TLC spots of lemon yellow,sunset yellow, orange G,acid orangeⅠ,gold orangeⅡ,auramine O,metanil yellow,basic orange 21 and basic orangeⅡwere clear and well sep-arated without interference from negative control. The limits of detection were 0.30,0.20,0.33,0.16,0.19,0.19,0.31,0.26, 0.30 mg/kg. CONCLUSIONS:The established method is able to detect adulterated dye and orange-yellow dye in Typhae Pollen rap-idly and accurately.
