Content Determination of Isoniazid in Isoniazid Tablets by Microfluidic Chip with Contactless Conductivi-ty Method
10.6039/j.issn.1001-0408.2017.09.25
- VernacularTitle:微流控芯片非接触电导法测定异烟肼片中异烟肼的含量
- Author:
Yanli TONG
;
Hanzhi FENG
;
Zuanguang CHEN
- Keywords:
Microfluidic chip;
Contactless conductivity detection;
Isoniazid;
Isoniazid tablet;
Content determination
- From:
China Pharmacy
2017;28(9):1242-1245
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE:To establish a method for the content determination of isoniazid in Isoniazid tablet. METHODS:Mi-crofluidic chip with contactless conductivity detection was adopted. The effects of electrophoresis parameters,such as the variety, concentration,pH,additive types and dosage,injection time and separation voltage on the separation detection were explored. RE-SULTS:The optimized buffer system was 1.86 mmol/L citrate-0.14 mmol/L sodium citrate buffer solution (pH3.0),no additive , the injection time was 20 s and the separation voltage was 2.5 kV. In the optimal conditions,the linear range of isoniazid was 10-200 μg/mL(r=0.9993);the limits of quantification and detection were 8.0 μg/mL and 2.4 μg/mL;RSDs of precision,stability and reproducibility tests were lower than 2.0%;recovery was 97.8%-102.9%(RSD=1.9%,n=9). CONCLUSIONS:The method is rapid and simple,and suitable for the content determination of isoniazid in Isoniazid tablet.
