Study on Improving the Quality Standard of Gubi Zhitong Liquor
10.6039/j.issn.1001-0408.2017.09.27
- VernacularTitle:骨痹止痛液的质量标准提高研究
- Author:
Xixiang LI
;
Xingyong LI
;
Qiang BAO
;
Xuemei WANG
- Keywords:
Guibi zhitong liquor;
Imperatorin;
Cinnamaldehyde;
TLC;
HPLC;
Quality standard
- From:
China Pharmacy
2017;28(9):1249-1253
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE:To improve the quality standard for Guibi zhitong liquor. METHODS:TLC was used for the qualita-tive identification of Radix angelicae,Notopterygium incisum,Radix aucklandiae and Magnolia officinalis in the preparation;HPLC was used for the contents determination of imperatorin and cinnamaldehyde:the column was Waters Symmetry Shield RP-C18 with mobile phase of methanol-water for imperatorin(60:40,V/V)and methanol-water for cinnamaldehyde(35:65,V/V)at a flow rate of 1.0 mL/min,detection wavelength was 254 nm for imperatorin and 290 nm for cinnamaldehyde,column temperature was 25℃,and the injection volume was 20μL. RESULTS:The TLC spots of R. angelicae,N. incisum,R. aucklandiae and M. of-ficinalis were clear and well separated,negative control without interference. The linear range was 3.0-30.0 μg/mL for imperatorin (r=0.9998)and 3.978-39.78 μg/mL for cinnamaldehyde(r=0.9999);RSDs of precision,stability and reproducibility tests were lower than 2.0%;recoveries were 96.94%-102.64%(RSD=2.37%,n=6)and 96.78%-99.53%(RSD=1.00%,n=6). CONCLU-SIONS:The improved standard more effectively control the quality of the Guibi zhitong liquor.
