Correlation between Toll-like receptor 4 in peripheral blood monocytes and asthma predictive index in wheezing children under 5 years of age
10.3969/j.issn.1671-8348.2017.08.002
- VernacularTitle:5岁以下喘息患儿哮喘预测指数与外周血单核细胞TLR4表达的相关性研究
- Author:
Zhen JIANG
;
Weihua LI
;
Feng ZHU
;
Haiyan WEI
;
Gongjian QI
- Keywords:
Toll-like receptor 4;
interleukin-6;
asthma
- From:
Chongqing Medicine
2017;46(8):1012-1014
- CountryChina
- Language:Chinese
-
Abstract:
Objective To study the expression of Toll-like receptor 4(TLR4) in peripheral blood mononuclear cell (PBMCs) and serum interleukin-6(IL-6) in wheezing children under 5 years of age.Methods A total of 224 wheezing children under 5 years of age were divided into API(asthma predictive index)-positive (n=116) and API-negative groups (n=108).Serum level of IL-6 and TLR4 expression on CD14+ monocytes were measured after wheezing was stable for one month.TLR4 expression on CD14+ monoeytes was quantified via flow-cytometry.Serum level of IL-6 was detected by ELISA.Results Serum level of IL-6 and TLR4 expression on CD14+ monocytes of API-positive group were higher than API-negative group [LR4(％):34.9±10.0 vs.30.2± 8.8;IL-6(ng/L):46.4±15.1 vs.40.5±13.6].There was a significant positive correlation between the expression of TLR4 and the content of serum IL-6 in two groups of wheezing children(P＜0.05).Conclusion TLR4 may play a role in the pathogenesis of asthma through promoting the expression of IL-6.TLR4 may be a index to predicting asthma in wheezing children.
