Establishment of human gastric cancer model in normal immune mice based on micro-carrier 6
10.3969/j.issn.1000-8179.2017.05.168
- VernacularTitle:基于microcarrier 6构建正常免疫小鼠人胃癌移植模型
- Author:
Yanzhen BI
;
Lingbin KONG
;
Pengfei GAO
;
Quanyi WANG
;
Yonghong YANG
;
Xiaobei ZHANG
;
Zeng FAN
;
Quanquan WANG
;
Bingcheng HUANG
;
Feng YANG
;
Qiusheng ZHANG
;
Yibo WANG
;
Fuqiang SUN
;
Ye HONG
;
Feng HONG
- Keywords:
gastric cancer;
microcarrier;
MKN45 cell;
animal model
- From:
Chinese Journal of Clinical Oncology
2017;44(5):199-203
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To establish a mouse model of gastric cancer by inoculating MKN45 cells into mice with normal immune function utilizing microcarrier technology. Methods:A total of 60 male C57BL/6 mice were randomly divided into three groups, namely, 2D, con-trol, and 3D groups, according to the coculture system of MKN45 and microcarrier. The mouse models of gastric carcinoma were estab-lished by hypodermic injection. The time of tumorigenesis, rate of tumor formation, and pathological features were observed in each group. Results:In the 3D group, the time of tumor formation was short, whereas the rate of tumor formation was high (80%). No de-tectable tumor formations were observed in the 2D and control groups. HE and immunohistochemical staining of the transplantation tumor model showed evident characteristics of human gastric cancer. Conclusion:A human gastric cancer model in normal immune mice was successfully established. The onset and development mechanism of gastric cancer could be more effectively investigated in mice with normal immune function through this model. Moreover, a more valuable and new animal model for the research and devel-opment of anticancer drug was established.
