Preliminary Study on the Effect of 5-Aza-CdR on the Demethylation of RNF180 in Prostate Cancer Cell Line DU145
10.12007/j.issn.0258-4646.2017.02.010
- VernacularTitle:5-Aza-CdR对DU145前列腺癌细胞系RNF180基因去甲基化作用的初步研究
- Author:
Haiguang WANG
;
Huamao JIANG
;
Zhirun ZUO
;
Huangzhe LONG
;
Guanyuan YUAN
;
Fanzhen JIA
- Keywords:
5-Aza-CdR;
prostate cancer;
RNF180;
demethylation
- From:
Journal of China Medical University
2017;46(2):140-144
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the mechanism and cause of the inactivation of tumor suppressor gene RNF180 in prostate cancer cell line by observing the effect of 5-Aza-CdR on the RNF180 gene in prostate cancer cell line DU145. Methods MTT method was adopted to study the effect of 5-Aza-CdR(0,1,2,5,10,15 and 20μmoI/L)on the proliferation of prostate cancer cells. Western blotting,real-time PCR,and methyla-tion specific PCR(MSP)were separately used to detect the expression of RNF180 in prostate cancer cells before and after the treatment of the most suitable drug concentration(5μmoI/L). Results In a certain range,the effect of 5-Aza-CdR on the proliferation of prostate cancer cell line DU145 was increased with the increase of drug concentration and the time of drug treatment(P<0.05). After the treatment of the most suitable drug concentration,the protein and mRNA expression of RNF180 in prostate cancer cells was significantly increased(P<0.05),but the methyla-tion of the promoter region was obviously decreased. Conclusion 5-Aza-CdR can reverse the methylation status of RNF180 gene in DU145 pros-tate cancer cell line,and relieve the silencing status of RNF180gene expression.
