Calycosin Enhance the Inhibitory Effect of Cisplatin on Proliferation of Gastric Cancer Cells

VernacularTitle:毛蕊异黄酮增强顺铂对胃癌细胞增殖的抑制作用
Author: Lixia ZHOU ; Yueming WU ; Na LI ; Yi YANG ; Xueyun QIN
Keywords: calycosin; cisplatin; BGC823; gastric; proliferation
From: Journal of China Medical University 2017;46(1):50-54
CountryChina
Language:Chinese
Abstract: Objective To observe the effect of calycosin on cisplatin?induced inhibition of human gastric BGC823 cells. Methods BGC823 cells were treated with different drugs:saline,cisplatin,calycosin and cisplatin combined with calycosin. MTT assays were used to detect the prolif?eration rate of BGC823 cell. Then the protein level and RNA level of cyclin D1,CDK4 and CDK6 were detected by Western blotting and Real?time PCR. Results The proliferation inhibition rates of BGC823 cells treated with 20μg/mL cisplatin,10μg/mL meperoflavone,and combina?tion of the 2 drugs were 56.44%± 2.08%,9.52%± 2.77%and 74.44%± 0.82%,respectively. The inhibition rate of the combination of drugs was significantly higher than that of the single drug treatment group(P<0.05). In addition,we found that calycosin can significantly enhance the inhi?bition of Cyclin D1,CDK4 and CDK6 by cisplatin in protein level and RNA level. Conclusion Calycosin can significantly increase the inhibitory rate of cisplatin on BGC823 cell proliferation,and the combination of the two drugs can reduce the side effects of cisplatin.